Big data's contribution to the development of Internet enterprises has enabled leading Internet platform service providers to implement competition-restrictive behaviors in terms of market access, bargaining rules, service provision, and data sharing. The monopolistic behavior implemented by Internet enterprises using big data presents new features such as algorithmic conspiracy, abuse of data dominance and cross-platform operator concentration. Based on the new development of Internet technology in the context of big data application, it is necessary to establish an anti-monopoly regulatory framework for Internet enterprises in terms of reaching monopoly agreements, recognizing the abuse of dominant market position, reviewing the concentration of operators, and derogating from consumer welfare. Under the concept of inclusive and prudent regulation, the focus of antitrust should be on the compliant operation of Internet enterprises. The theory of relative advantage should be introduced to further regulate the market competition behavior of the head enterprises, and the criteria for evaluating the impact of actual competition should be constructed. The rights and obligations of Internet enterprises of different levels in market competition should be clarified in accordance with the requirements of hierarchical classification and the relevant supporting regulatory measures should be improved. The system for sharing big data in the era of digital economy should be established and perfected. It is also necessary to establish a system of symbiosis and sharing of big data, and to take into account the antitrust and personal information protection of big data, so as to promote the improvement of consumer welfare.