Based on the linkages between headquarters and branches of high-growth enterprises in Jiangsu, this paper constructs inter--city enterprise networks through the Interlocking Network Model, and analyzes the spatio-temporal evolution characteristics of inter-city high-growth enterprise networks of Jiangsu through the lens of enterprise layout, network indexes, and spatial connections. We find that: (1) The high-growth enterprises show great spatial concentration with more than 70% headquarters located in Suzhou and Nanjing, and branches located in major urban agglomerations and provincial capitals. From 2018 to 2020, the number of headquarters in Nanjing increased rapidly whereas the growth of Suzhou slowed down, and the headquarter economy in North Jiangsu rose. The branches expanded from coastal to inland cities. (2) The high-growth enterprise network indexes of Suzhou and Nanjing ranked the 1st and 2nd, and the gap between them became smaller. During then, Suzhou's high-growth enterprises showed an inverted U-shaped development; Nanjing maintained a steady upward trend but Zhenjiang and Yancheng dropped precipitously. The inter-city gap in Jiangsu increased after the COVID-19 epidemic. In terms of sub-type indexes, Gazelle enterprises were most severely affected by the epidemic, while (cultivating) unicorn enterprises embraced a new development. The industry with the highest contribution is the information technology service industry. (3) The overall networks of Jiangsu's high-growth enterprises became denser, larger, and polycentric. However, their internal connections within the province were relatively sparse and decreased from south to north, thus provincial integration needs to be improved. On this basis, the paper proposes policy suggestions of optimizing enterprise layout, coordinating regional development, strengthening intra-provincial linkages, and so forth.