The informal information before an organizational change refers to unconfirmed information through informal channels. Such information may lead to employees’ resistance to change. To explore the influence of negative informal information, we investigated the negative informal information on employee initial resistance. We examined the change of employee resistance over time as well as the moderating roles of conscientiousness and negative informal information frequency on the change of employee resistance to organization change. Based on 159 employees’ data analysis the results demonstrated that (1) the content of negative informal information before change positively related to the employees’ resistance to change, and the resistance to change of employees attenuated significantly over time; (2) under the low frequency of negative informal information, the resistance to change of employees with low responsibility decreases significantly over time. The research results can guide the implementation of reform practice in China.