By combining the business ecosystem theory with the virtual business circle, this article tries to explain the static and dynamic evolution of the internal structure of the virtual business circle. On this basis, it conducts an in-depth exploration into the internal mechanism and operation mechanism of the development of virtual internal ecological district from the theoretical angle, and simulates the internal evolution of the virtual business circle of ecological system with computer simulation. This study finds: the virtual evolution of the ecological structure has experienced the four main stages of germination period, growth period, breeding period, and differentiation stage, and the business development has gone from the three stages with the platforms, businesses, consumers as subjects to the stage of five subjects, including the platforms, businesses, consumers, professional service providers, and value-added service providers. They evolve and breed to generate new professional service businesses and value-added service businesses, resulting in a new virtual business circle. Finally, taking Alibaba e-commerce ecosystem as a case study, this paper analyzes the development and evolution of its internal ecosystem.